1 August 2003 Spectral staining of tumor tissue by fiber optic FTIR spectroscopy
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Infrared (IR) optical fiber have aroused great interest in recent years because of their potential in in-vivo spectroscopy. This potential includes the ability to be flexible, small and to guide IR light in a very large range of wavelengths. Two types - silver halide and chalcogenide - infrared transmitting fibers are investigated in the detection of a malignant tumor. As a test sample for all types of fibers we used a thin section of an entire rat brain with glioblastoma. The fibers were connected with a common infrared microscope. Maps across the whole tissue section with more than 200 spectra were recorded by moving the sample with an XY stage. Data evaluation was performed using fuzzy c-means cluster analysis (FCM). The silver halide fibers provided excellent results. The tumor was clearly discernible from healthy tissue. Chalcogenide fibers are not suitable to distinguish tumor from normal tissue because the fiber has a very low transmittance in the important fingerprint region.
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Reiner Salzer, Reiner Salzer, Gerald Steiner, Gerald Steiner, Angelique Kano, Angelique Kano, Tom Richter, Tom Richter, Ralf Bergmann, Ralf Bergmann, Heike Rodig, Heike Rodig, Bernd Johannsen, Bernd Johannsen, Jens Kobelke, Jens Kobelke, } "Spectral staining of tumor tissue by fiber optic FTIR spectroscopy", Proc. SPIE 5047, Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II, (1 August 2003); doi: 10.1117/12.484153; https://doi.org/10.1117/12.484153

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