Planar MEMS bio-chip for recording ion-channel currents in biological cells

Santosh Pandey; Zannatul Ferdous; Marvin H. White

doi:10.1117/12.514745

14 October 2003 Planar MEMS bio-chip for recording ion-channel currents in biological cells

Santosh Pandey, Zannatul Ferdous, Marvin H. White

Proceedings Volume 5062, Smart Materials, Structures, and Systems; (2003) https://doi.org/10.1117/12.514745
Event: Smart Materials, Structures, and Systems, 2002, Bangalore, India

Abstract

We describe a planar MEMS silicon structure to record ion-channel currents in biological cells. The conventional method of performing an electrophysiological experiment, 'patch-clamping,' employs a glass micropipette. Despite careful treatments of the micropipette tip, such as fire polishing and surface coating, the latter is a source of thermal noise because of its inherent, tapered, conical structure, which gives rise to a large pipette resistance. This pipette resistance, when coupled with the self-capacitance of the biological cell, limits the available bandwidth and processing of fast transient, ion channel current pulses. In this work, we reduce considerably the pipette resistance with a planar micropipette on a silicon chip to permit the resolution of sub-millisecond, ion-channel pulses. We discuss the design topology of the device, describe the fabrication sequence, and highlight important critical issues. The design of an integrated on-chip CMOS instrumentation amplifier is described, which has a low-noise front-end, input-offset cancellation, correlated double sampling (CDS), and an ultra-high gain in the order of 10¹²V/A.

Citation Download Citation

Santosh Pandey, Zannatul Ferdous, and Marvin H. White "Planar MEMS bio-chip for recording ion-channel currents in biological cells", Proc. SPIE 5062, Smart Materials, Structures, and Systems, (14 October 2003); https://doi.org/10.1117/12.514745

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