The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe cultured at 37°C

Vladimir N. Korsukov; Tatyana N. Schukovskaya; Svetlana N. Klueva; Alexander L. Kravtsov; Tatyana A. Polunina; Youri A. Popov

doi:10.1117/12.518632

13 October 2003 The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe cultured at 37°C

Vladimir N. Korsukov, Tatyana N. Schukovskaya, Svetlana N. Klueva, Alexander L. Kravtsov, Tatyana A. Polunina, Youri A. Popov

Author Affiliations +

Proceedings Volume 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV; (2003) https://doi.org/10.1117/12.518632
Event: Saratov Fall Meeting 2002 Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III, 2002, Saratov, Russian Federation

Abstract

The results have been obtained by flow cytometry showed the significant raise of fluorescence intensity of Yersinia pestis EV cells binding FITC-labelled plague polyclonal immunoglobins after 24 h cultivation in Hottinger broth pH 7.2 at 37 °C in the presence of increasing concentration serotonin (5-Hydroxytryptamine). The presence of serotonin (5-HT) in nutrient broth at concentration of 10^-5M, 10^-8M could modulate DNA content in 37 °C growing population of plague microbe. The effect of this action depended of 5-HT concentration.

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Vladimir N. Korsukov, Tatyana N. Schukovskaya, Svetlana N. Klueva, Alexander L. Kravtsov, Tatyana A. Polunina, and Youri A. Popov "The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe cultured at 37°C", Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); https://doi.org/10.1117/12.518632

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