15 August 2003 Hybridization assay based on evanescent fluorescence excitation and collection
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Abstract
There is a great need for high throughput and sensitive sensors for genetic analysis. These sensors can be used for varied purposes from monitoring gene expression in organims to speciation of possible pathogens. Consequently, an instrument capable of these tasks would be a great benefit for food and water safety, medical diagnostics and defense of military and civilian populations from biological threats. This work examines the development of a hybridization-based biosensor using a novel tapered fiber optic rpobe. The immobilization of single-stranded, synthetic ologinucleotides utilizing aminoproplytriethoxysilane and glutaraldehyde was implemented on the fiber optic sensor. Hybridization takes place with a complementary analyte sequence followed by a fluorescent, labeled signaling probe to form a sandwich assay. Following hybridization, the fiber is interrogated with a diode laser source and the resulting fluorescence signal is detected using a miniature spectrometer.
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James J. Sumner, Robert U. Mmerole, Dimitra N. Stratis-Cullum, Hyunmin Yi, William E. Bentley, James B. Gillespie, "Hybridization assay based on evanescent fluorescence excitation and collection", Proc. SPIE 5085, Chemical and Biological Sensing IV, (15 August 2003); doi: 10.1117/12.486963; https://doi.org/10.1117/12.486963
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