Three-dimensional localization of fluorescent spots with adapted MUSIC algorithm

Bernhard Scholz; Marcus Pfister

doi:10.1117/12.500473

15 October 2003 Three-dimensional localization of fluorescent spots with adapted MUSIC algorithm

Bernhard Scholz, Marcus Pfister

Proceedings Volume 5138, Photon Migration and Diffuse-Light Imaging; (2003) https://doi.org/10.1117/12.500473
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

We present a novel method, space-space MUSIC (MUltiple SIgnal Classification), to localize three-dimensionally focal fluorophore-tagged lesions activated subsequently by different laser source posi-tions from multi-sensor fluorescence data obtained from a single measurement plane. Matches between a signal subspace derived from the measured data and data from model spots allow 3D determination of the centers-of-gravity of fluorescence regions. Simulated spots in bounded, inho-mogeneous media could be localized accurately. The algorithm has shown to be robust against patient-dependent parameters, such as optical background parameters. The algorithm does also not consider medium boundaries.

Citation Download Citation

Bernhard Scholz and Marcus Pfister "Three-dimensional localization of fluorescent spots with adapted MUSIC algorithm", Proc. SPIE 5138, Photon Migration and Diffuse-Light Imaging, (15 October 2003); https://doi.org/10.1117/12.500473

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