9 October 2003 CARS microscopy with dark field excitation
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We use coherent anti-Stokes Raman scattering (CARS) for functional imaging in microscopy. In contrast to other methods, excitation and detection are performed in a wide-field (non-confocal) setup, similar to a combination of dark field and epi-fluorescence microscopy. Thus, imaging of the whole sample is performed at once, i.e. without scanning, which promises the possibility of fast microscopy with vibrational contrast. The use of a nanosecond laser system rather than typically used pico- or femtosecond systems facilitates a high spectroscopic resolution for various organic substances.
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Christoph Heinrich, Christoph Heinrich, Stefan Bernet, Stefan Bernet, Monika Ritsch-Marte, Monika Ritsch-Marte, } "CARS microscopy with dark field excitation", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.499634; https://doi.org/10.1117/12.499634

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