Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope

Sheng Fu; Teck-Chee Chia; Leong Chuan Kwek; Cheong Hoong Diong; Choong Leong Tang; Francis Seow Choen; Shankar Muthu Krishnan

doi:10.1117/12.500203

9 October 2003 Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope

Sheng Fu, Teck-Chee Chia, Leong Chuan Kwek, Cheong Hoong Diong, Choong Leong Tang, Francis Seow Choen, Shankar Muthu Krishnan

Author Affiliations +

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500203
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

We investigated normal and cancerous human colorectal tissues (fresh thick biopsy specimens) using Olympus Confocal laser scanning biological microscope (FV300). The different layers of autofluorescence images of the specimen were captured by 488 nm laser scanning and sectioning. Optical sectioning can be performed in the vertical plane. Laser scanning can be performed in the horizontal plane. By comparing the autofluorescence image of the normal colorectal tissue with cancerous tissue, the structures of the optical sectioning image layer were found to be significantly different. We have also obtained fibrous autofluorescence image inside tissue specimen. Our investigation may help provide some useful insight to other autofluorescence research studies like laser induced autofluorescence spectra of human colorectal tissue study as a diagnosis technique for clinical application.

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Sheng Fu, Teck-Chee Chia, Leong Chuan Kwek, Cheong Hoong Diong, Choong Leong Tang, Francis Seow Choen, and Shankar Muthu Krishnan "Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500203

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