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9 October 2003 Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope
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Abstract
We investigated normal and cancerous human colorectal tissues (fresh thick biopsy specimens) using Olympus Confocal laser scanning biological microscope (FV300). The different layers of autofluorescence images of the specimen were captured by 488 nm laser scanning and sectioning. Optical sectioning can be performed in the vertical plane. Laser scanning can be performed in the horizontal plane. By comparing the autofluorescence image of the normal colorectal tissue with cancerous tissue, the structures of the optical sectioning image layer were found to be significantly different. We have also obtained fibrous autofluorescence image inside tissue specimen. Our investigation may help provide some useful insight to other autofluorescence research studies like laser induced autofluorescence spectra of human colorectal tissue study as a diagnosis technique for clinical application.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sheng Fu, Teck-Chee Chia, Leong Chuan Kwek, Cheong Hoong Diong, Choong Leong Tang, Francis Seow Choen, and Shankar Muthu Krishnan "Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500203
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