Multiphoton microscopy at an excitation wavelength of 1.26 µm

Karsten Plamann; Gilbert Boyer; Denis Chemla

doi:10.1117/12.500487

9 October 2003 Multiphoton microscopy at an excitation wavelength of 1.26 μm

Karsten Plamann, Gilbert Boyer, Denis Chemla

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500487
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

We present a multiphoton microscope using a Cr⁴⁺:forsterite femtosecond laser with an emission wavelength of 1260 nm for the excitation of the multiphoton processes. This wavelength is well adapted to the "optical window" in biological tissues and permits to reach higher imaging depths than systems using more conventional titanium:sapphire laser sources. The paper describes the experimental set-up and reports on first results on human cornea and skin samples.

Citation Download Citation

Karsten Plamann, Gilbert Boyer, and Denis Chemla "Multiphoton microscopy at an excitation wavelength of 1.26 μm", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500487

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