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9 October 2003 Time-resolved multifocal multiphoton microscopy
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Abstract
Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long time acquisition for 3D images. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition and gives both intensity and lifetime images for all multifocal points.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sandrine Leveque-Fort, J. Penon, Marie-Pierre Fontaine-Aupart, Gerard Roger, and Patrick Georges "Time-resolved multifocal multiphoton microscopy", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500627
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