9 October 2003 Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways
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Abstract
Using fluorescence resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM) we have explored the protein-protein interactions between fluorescent protein tagged fusion proteins of the activation pathways of PKC and NFkB. We observe FRET between CFP-IκB and YFP-p65 in unstimulated cells and when treated with TNFα. We also observed a reduction of the fluorescent lifetime of CFP-IκB in the absence of YFP-p65 when TNFα is present.
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Penny E. Morton, Tony Ng, Sarah A. Roberts, Borivoj Vojnovic, Simon M. Ameer-Beg, "Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.500513; https://doi.org/10.1117/12.500513
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