Time-resolved multiphoton imaging of the interaction between the PKC and the NFkappaB cell signalling pathways

Penny E. Morton; Tony Ng; Sarah A. Roberts; Borivoj Vojnovic; Simon M. Ameer-Beg

doi:10.1117/12.500513

9 October 2003 Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways

Penny E. Morton; Tony Ng; Sarah A. Roberts; Borivoj Vojnovic; Simon M. Ameer-Beg

Author Affiliations +

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500513
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Using fluorescence resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM) we have explored the protein-protein interactions between fluorescent protein tagged fusion proteins of the activation pathways of PKC and NFkB. We observe FRET between CFP-IκB and YFP-p65 in unstimulated cells and when treated with TNFα. We also observed a reduction of the fluorescent lifetime of CFP-IκB in the absence of YFP-p65 when TNFα is present.

Citation Download Citation

Penny E. Morton, Penny E. Morton, Tony Ng, Tony Ng, Sarah A. Roberts, Sarah A. Roberts, Borivoj Vojnovic, Borivoj Vojnovic, Simon M. Ameer-Beg, Simon M. Ameer-Beg, } "Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.500513; https://doi.org/10.1117/12.500513

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