Time-resolved multiphoton imaging of the interaction between the PKC and the NFkappaB cell signalling pathways

Penny E. Morton; Tony Ng; Sarah A. Roberts; Borivoj Vojnovic; Simon M. Ameer-Beg

doi:10.1117/12.500513

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9 October 2003 Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways

Penny E. Morton; Tony Ng; Sarah A. Roberts; Borivoj Vojnovic; Simon M. Ameer-Beg

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Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500513
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Using fluorescence resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM) we have explored the protein-protein interactions between fluorescent protein tagged fusion proteins of the activation pathways of PKC and NFkB. We observe FRET between CFP-IκB and YFP-p65 in unstimulated cells and when treated with TNFα. We also observed a reduction of the fluorescent lifetime of CFP-IκB in the absence of YFP-p65 when TNFα is present.

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Penny E. Morton, Tony Ng, Sarah A. Roberts, Borivoj Vojnovic, and Simon M. Ameer-Beg "Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500513

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