14 October 2003 New method for confocal microscopy and its endoscopic application
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Abstract
Today, confocal microscopy is widely used in biomedical applications, since its technology allows e.g. in cell biology to resolve sub- and intercelluar details in three-dimensions. Moreover, the recent miniaturization process of opto-electronical components even indicates a possible endoscopic application - to intraoperative examinations without need for a surgical biopsy. The current development of such a confocal endomicroscope, based on the concept of miniaturized fiberoptical components is described. The technical setup is explained in the context of an alternative method for confocal microscopy employing a Digital Micromirror Device (DMD). Moreover, in-vivo tissue staining techniques suitable for confocal endoscopic imaging are analyzed.
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Ingo Krohne, Tilo Pfeifer, Frank Bitte, Michael Zacher, Richard Meier, "New method for confocal microscopy and its endoscopic application", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); doi: 10.1117/12.500763; https://doi.org/10.1117/12.500763
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