X-ray microscopy and imaging of Caenorhabditis elegans nematode using a laser-plasma-pulsed x-ray source

Giulio Poletti; Franceasco Orsini; Jiri Ullschmied; Jiri Skala; Bozena Kralikova; Miroslav Pfeifer; Christelle Kadlec; Tomas Mocek; A. R. Prag; F. Cotelli; C. Lora Lamia; Dimitri Batani; A. Bernardinello; Tara Desai; A. Zullini

doi:10.1117/12.508377

7 January 2004 X-ray microscopy and imaging of Caenorhabditis elegans nematode using a laser-plasma-pulsed x-ray source

Giulio Poletti, Franceasco Orsini, Jiri Ullschmied, Jiri Skala, Bozena Kralikova, Miroslav Pfeifer, Christelle Kadlec, Tomas Mocek, A. R. Prag, F. Cotelli, C. Lora Lamia, Dimitri Batani, A. Bernardinello, Tara Desai, A. Zullini

Author Affiliations +

Proceedings Volume 5196, Laser-Generated and Other Laboratory X-Ray and EUV Sources, Optics, and Applications; (2004) https://doi.org/10.1117/12.508377
Event: Optical Science and Technology, SPIE's 48th Annual Meeting, 2003, San Diego, California, United States

Abstract

An experiment on Soft X-ray Contact Microscopy (SXCM) performed on Caenorhabditis elegans nematodes is discussed. This sample has been selected since it is a well studied case used as model in many biological contexts. The experiment has been performed using the iodine PALS laser source to generate pulsed soft X-rays from laser-plasma interaction, using molybdenum and gold as targets. Typical intensities on the targets exceeded 10¹⁴ W/cm². The SXCM imprints have been recorded on Polymethilmetacrylate (PMMA) photo resists which have been chemically developed and analyzed with an Atomic Force Microscope (AFM) operating in constant force mode. The use of error signal AFM images together with topography AFM images, did allow an easier recognition of biological patterns, and the identification of observed structures with internal organs. Several organs were identified in the SXCM images, including cuticle annuli, alae, pharynx, and three different types of cell nuclei. These are the first SXCM images of multi-cellular complex organisms.

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Giulio Poletti, Franceasco Orsini, Jiri Ullschmied, Jiri Skala, Bozena Kralikova, Miroslav Pfeifer, Christelle Kadlec, Tomas Mocek, A. R. Prag, F. Cotelli, C. Lora Lamia, Dimitri Batani, A. Bernardinello, Tara Desai, and A. Zullini "X-ray microscopy and imaging of Caenorhabditis elegans nematode using a laser-plasma-pulsed x-ray source", Proc. SPIE 5196, Laser-Generated and Other Laboratory X-Ray and EUV Sources, Optics, and Applications, (7 January 2004); https://doi.org/10.1117/12.508377

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KEYWORDS

X-rays

Plasma

X-ray microscopy

Atomic force microscopy

Polymethylmethacrylate

Gold

Molybdenum

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