Novel fluorophores for single-molecule imaging

Katherine A. Willets; Oksana Ostroverkhova; Stephan Hess; Meng He; Robert J Twieg; William E. Moerner

doi:10.1117/12.506519

14 November 2003 Novel fluorophores for single-molecule imaging

Katherine A. Willets, Oksana Ostroverkhova, Stephan Hess, Meng He, Robert J Twieg, William E. Moerner

Proceedings Volume 5222, Nanocrystals, and Organic and Hybrid Nanomaterials; (2003) https://doi.org/10.1117/12.506519
Event: Optical Science and Technology, SPIE's 48th Annual Meeting, 2003, San Diego, California, United States

Abstract

A new class of fluorophores has been identified that can be imaged at the single-molecule level and offer additional beneficial properties such as a significant ground state dipole moment, moderate hyperpolarizability, and sensitivity to local rigidity. These molecules contain an amine donor and a dicyanodihydrofuran (DCDHF) acceptor linked by a conjugated unit (benzene, thiophene, alkene, styrene, etc.) and were originally designed to deliver both high polarizability anisotropy and dipole moment as nonlinear optical chromophores for photorefractive applications. Surprisingly, we have found that these molecules are also well-suited for single-molecule fluorescence imaging in polymers and other reasonably rigid environments. We report the bulk (ensemble) and single-molecule photophysical properties measured for six dyes in this new class of single-molecule reporters, with absorption maxima ranging from 486 to 614 nm.

Citation Download Citation

Katherine A. Willets, Oksana Ostroverkhova, Stephan Hess, Meng He, Robert J Twieg, and William E. Moerner "Novel fluorophores for single-molecule imaging", Proc. SPIE 5222, Nanocrystals, and Organic and Hybrid Nanomaterials, (14 November 2003); https://doi.org/10.1117/12.506519

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