Determination and description of groundwater systems is essential for the management and development of ecological values, especially in the valley parts of river basins. At the land surface, groundwater systems appear as infiltration (relatively dry) and discharge zones (relatively wet). Groundwater discharge zones offer a high potential for nature values because of their constant moisture presence and their specific water quality. Current methods for the determination of discharge and infiltration zones use either detailed time-consuming fieldwork or data intensive numerical simulation models. Consequently, there is a direct need for repeatable, area covering, mapping possibilities for the determination of moisture gradients and more specifically discharge and infiltration zones. Within the framework of the CASI-SWIR measuring campaign 2002, the Department of Hydrology and Hydraulic Engineering of the Vrije Universiteit Brussel (VUB) executed an airborne hyperspectral remote sensing and field campaign in the Doode Bemde to analyze moisture gradients in the Doode Bemde, a riparian nature reserve. The main objective of the study is to test the best hyperspectral analysis method, using the hyperspectral CASI-SWIR data, for the known, based upon field and simulation data, moisture gradients in the Doode Bemde area. Simultaneously with the airborne hyperspectral campaign, field measurements of soil moisture, groundwater levels, vegetation temperature and spectral characteristics of some key vegetation species (phreatophytes) were performed. The method of analysis consists of statistical comparison of moisture gradients, obtained from measurements and simulations, with individual bands, a combination of bands and multivariate derivatives. The paper describes the set-up of the field and airborne measurement campaign, the methodology of analysis as well as first analysis results.