18 February 2004 Using fiber optic probe and CMOS BDJ detector for microarray spot scanning
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Proceedings Volume 5249, Optical Design and Engineering; (2004); doi: 10.1117/12.512503
Event: Optical Systems Design, 2003, St. Etienne, France
Abstract
We have designed a cost-effective, fiber optic bundle-based detection system for microarray fluorescence measurements. A bundle, fabricated with thin-cladding fibers of 50-μm in core diameter, is used for spot excitation and collection. The collected optical signal is detected by a CMOS BDJ (Buried Double p-n Junction) detector, which can be operated either as a photodiode or as a wavelength-sensitive device. For improving measuring rate of a microarray, we have proposed a direct spot scanning technique, which is based on a prior knowledge about the predefined microarray's mask pattern, and operates to bring the bundle successively over each spot for single-point measurement. It is implemented with a microarray registration procedure to determine the spots' positions. The detection system with implemented scanning technique has been tested using microarray samples. Its scanning operation has been verified by comparing the determined spots' coordinates to the microarray image.
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Patrick Pittet, Loubna Hannati, Guo Neng Lu, "Using fiber optic probe and CMOS BDJ detector for microarray spot scanning", Proc. SPIE 5249, Optical Design and Engineering, (18 February 2004); doi: 10.1117/12.512503; https://doi.org/10.1117/12.512503
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KEYWORDS
CMOS sensors

Luminescence

Sensors

Fiber optics

Signal detection

Image resolution

Image registration

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