25 February 2004 Optical thin films serving biotechnology: fluorescence enhancement of DNA-chip
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Abstract
Over the past years, DNA-chip technology has exploded. Yet scientists using such devices have to face many problems. One of them, due to the very low concentration of biological species to be detected, is the weakness of fluorescence signal collected through the reading system (microscope or scanner). To solve this problem, we proposed to use optical thin films technology. We studied the potentialities of this method step by step. The first step was to be able to understand, explain and forecast the fluorescence emitted by a DNA-chip in terms of fluorescence angular patterns. A theoretical and experimental study enabled us to master this issue even in the case of multi-layers substrates. Using this knowledge we were then able to explain, through simulations, the potentialities of this new type of substrates in terms of fluorescence enhancement. Thus we showed that a theoretical enhancement of twenty-fold (compare to a glass substrate) was achievable.
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Pierre Barritault, Pierre Barritault, Stephane Getin, Stephane Getin, Patrick Chaton, Patrick Chaton, Bernard Andre, Bernard Andre, Francoise Vinet, Francoise Vinet, Brigitte Fouque, Brigitte Fouque, } "Optical thin films serving biotechnology: fluorescence enhancement of DNA-chip", Proc. SPIE 5250, Advances in Optical Thin Films, (25 February 2004); doi: 10.1117/12.516261; https://doi.org/10.1117/12.516261
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