19 February 2004 CMOS optical detector system for capillary fluorescence measurements
Author Affiliations +
Proceedings Volume 5251, Detectors and Associated Signal Processing; (2004); doi: 10.1117/12.512407
Event: Optical Systems Design, 2003, St. Etienne, France
Abstract
We present a fluorescence detection system for capillary analysis. It is designed using a CMOS BDJ (Buried Double p-n Junction) detector which can be operated either as a photodiode or as a wavelength-sensitive device. Noise-reduction techniques such as signal pre-amplification and synchronous detection are implemented to boost the sensitivity of measurements. The system indicates fluorescence intensity for concentration determination, and average wavelength of fluorescence spectrum for molecular discrimination. The system has been tested by measuring two widely used fluorophores (FITC and Rhodamine B) in different concentrations. A 407-nm blue laser diode and a 532-nm green YAG compact laser have been respectively employed for their excitation. The illuminated volume inside the capillary is about 5 nl. The best results have been obtained with FITC, enabling as low as 10-10 M to be detectable.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jean-Marc Galvan, Guo-Neng Lu, Patrick Pittet, Loic Blum, Beatrice Leca-Bouvier, "CMOS optical detector system for capillary fluorescence measurements", Proc. SPIE 5251, Detectors and Associated Signal Processing, (19 February 2004); doi: 10.1117/12.512407; http://dx.doi.org/10.1117/12.512407
PROCEEDINGS
8 PAGES


SHARE
KEYWORDS
Luminescence

Sensors

Signal detection

Capillaries

CMOS sensors

Rhodamine B

Fluorescence spectroscopy

RELATED CONTENT

RPV Sensor System Interface
Proceedings of SPIE (September 08 1976)
An optofluidic FRET laser using quantum dots as donor
Proceedings of SPIE (April 22 2016)
Automatic laser refractometer
Proceedings of SPIE (November 04 2003)
Super-resolution imaging based on virtual Airy spot
Proceedings of SPIE (March 06 2015)
Design and fabrication of a debris flow sensor using a...
Proceedings of SPIE (January 30 2012)

Back to Top