19 February 2004 CMOS optical detector system for capillary fluorescence measurements
Author Affiliations +
Proceedings Volume 5251, Detectors and Associated Signal Processing; (2004); doi: 10.1117/12.512407
Event: Optical Systems Design, 2003, St. Etienne, France
We present a fluorescence detection system for capillary analysis. It is designed using a CMOS BDJ (Buried Double p-n Junction) detector which can be operated either as a photodiode or as a wavelength-sensitive device. Noise-reduction techniques such as signal pre-amplification and synchronous detection are implemented to boost the sensitivity of measurements. The system indicates fluorescence intensity for concentration determination, and average wavelength of fluorescence spectrum for molecular discrimination. The system has been tested by measuring two widely used fluorophores (FITC and Rhodamine B) in different concentrations. A 407-nm blue laser diode and a 532-nm green YAG compact laser have been respectively employed for their excitation. The illuminated volume inside the capillary is about 5 nl. The best results have been obtained with FITC, enabling as low as 10-10 M to be detectable.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jean-Marc Galvan, Guo-Neng Lu, Patrick Pittet, Loic Blum, Beatrice Leca-Bouvier, "CMOS optical detector system for capillary fluorescence measurements", Proc. SPIE 5251, Detectors and Associated Signal Processing, (19 February 2004); doi: 10.1117/12.512407; http://dx.doi.org/10.1117/12.512407



Signal detection


CMOS sensors

Rhodamine B

Fluorescence spectroscopy


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