8 December 2003 Design and application of fiber optic evanescent wave biosensor
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Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546217
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China
Abstract
A fiber-optic biosensor is developed based on the principle of evanescent wave while light propagates in optical fiber. The biosensor uses a red laser diode at 636.85 nm for exciting Cy5 fluorescent dye. Sensitivity limit of 0.01 nnmol/l is obtained from the detection of serial Cy5 solutions with various concentrations. In log-to-log plot, excellent linear response characteristic is seen in the Cy5 concentrations ranging from 0.01 nmlo/l to 100 nmol/l. And a good result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 μmol/l. All the results are comparable with those that are obtained by a commercial biochip scanner GeneTAC 1000.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Huijie Huang, Huijie Huang, Junhui Zhai, Junhui Zhai, Yongkai Zhao, Yongkai Zhao, Ruifu Yang, Ruifu Yang, Bingqiang Ren, Bingqiang Ren, Zhaogu Cheng, Zhaogu Cheng, Longlong Du, Longlong Du, Dunwu Lu, Dunwu Lu, } "Design and application of fiber optic evanescent wave biosensor", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546217; https://doi.org/10.1117/12.546217
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