8 December 2003 Investigation on the fluorescence spectra of thioredoxin reductase from human brain
Author Affiliations +
Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546563
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China
Abstract
The technique of fluorescence spectroscopy is applied to study thioredoxin reductast (TrxR) in the cells of human brain. Experimental results show that, by the ultraviolet light irradiation (λmax=253.7nm), TrxR is able to emit two striking spectral bands of 287nm to 484nm and 560nm to 720nm. The spectral profile also consists of some narrow spike-like bands atop these two broad bands. With the concentration of TrxR decreases, the narrow spike-like bands disappear little by little. Furthermore, physical and biochemical mechanisms of fluorescence production for ultraviolet light-induced TrxR spectra and its characteristics are analyzed. The new spectroscopic information suggested in this paper may represent an effort of better understanding of the structure and conformation changes of TrxR.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xiufeng Lan, Xiufeng Lan, Ying Liu, Ying Liu, Yunjing Ji, Yunjing Ji, Zhonghua Shen, Zhonghua Shen, Jian Lu, Jian Lu, Xiao-Wu Ni, Xiao-Wu Ni, Tao Yang, Tao Yang, Xiaosen Luo, Xiaosen Luo, Jiangang Liu, Jiangang Liu, Lin Xu, Lin Xu, "Investigation on the fluorescence spectra of thioredoxin reductase from human brain", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546563; https://doi.org/10.1117/12.546563
PROCEEDINGS
6 PAGES


SHARE
RELATED CONTENT

Bacteriorhodopsin as a chemical and biological sensor
Proceedings of SPIE (August 14 2003)
Site-selective spectroscopy of myoglobin
Proceedings of SPIE (June 16 1993)
Spectroscopic properties of tryptophan and bacteria
Proceedings of SPIE (April 11 1995)
Proton-transfer laser
Proceedings of SPIE (April 30 1992)

Back to Top