4 December 2003 Fluorescence correlation spectroscopy based upon two-photon excitation
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Proceedings Volume 5255, Biomolecular Photonics and Multidimensional Microscopy; (2003) https://doi.org/10.1117/12.546244
Event: Biomolecular Photonics and Multidimensional Microscopy, 2003, Wuhan, China
Abstract
Fluorescence correlation spectroscopy (FCS) is a powerful tool for measurement of biological dynamic processes. In this studying, a two-photon excitation fluorescence correlation spectroscopy (TP-FCS) system was set up depending on a part of optical block and detector of the multi-photon excitation fluorescence microscope (MPLFM). The phenomenon "photon-burst" was observed from the TP-FCS system. Meanwhile, the diffusion coefficient of rhodamine B molecule in sucrose aqueous solution was calculated. It was proved that TP-FCS is especially suited for integration into MPEFM accordingly to yield a hybride-technology with the peculiarities of the individual technique and the advantage of mutual synergistic effects. The fusion of both techniques seems to be reasonable and desirable to reduce costs.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yafeng Liu, Yafeng Liu, Tongsheng Chen, Tongsheng Chen, Qingming Luo, Qingming Luo, } "Fluorescence correlation spectroscopy based upon two-photon excitation", Proc. SPIE 5255, Biomolecular Photonics and Multidimensional Microscopy, (4 December 2003); doi: 10.1117/12.546244; https://doi.org/10.1117/12.546244
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