4 December 2003 The fluorescent photobleaching properties of GFP expressed in human lung cancer cells
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Proceedings Volume 5255, Biomolecular Photonics and Multidimensional Microscopy; (2003); doi: 10.1117/12.546245
Event: Biomolecular Photonics and Multidimensional Microscopy, 2003, Wuhan, China
Abstract
The characteristic properties of GFP make this protein a good candidate for use as a molecular reporter to monitor patterns of protein localization, gene expression, and intracellular protein trafficking in living cells. In this study, the dicistronic expression vector (pEGFP-C1) was used to transfected into human lung cancer cell line (ASTC-a-1) and a positive clone which stably expressed GFP in high level was obtained. After more than three months' passengers, the cells were also remained the strong fluorescence under fluorescent microscope. The results showed that the green fluorescent protein expressed in tumor cells was also photobleached under intense irradiation (approximately 488 nm) and the degree of photobleaching varied with the difference of the intensity of the excitation. Using different interdiction parcel (None, ND4, ND8, ND16), there were significant differences in photobleaching among the different excitation. The photobleaching was also affected by the time length of excitation, and the intensity of fluorescence was obviously decreased along with the increasing of excitation time, especially to stronger excitation.
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Ying Jin, Da Xing, "The fluorescent photobleaching properties of GFP expressed in human lung cancer cells", Proc. SPIE 5255, Biomolecular Photonics and Multidimensional Microscopy, (4 December 2003); doi: 10.1117/12.546245; https://doi.org/10.1117/12.546245
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