Kinetics of camptothecin hydrolysis determined by PCA and FA analysis of fluorescence spectra

Stefan Kruszewski; Ryszard Siuda; Blanka Ziomkowska; Michal Cyrankiewicz

doi:10.1117/12.545163

21 November 2003 Kinetics of camptothecin hydrolysis determined by PCA and FA analysis of fluorescence spectra

Stefan Kruszewski, Ryszard Siuda, Blanka Ziomkowska, Michal Cyrankiewicz

Proceedings Volume 5259, 13th Polish-Czech-Slovak Conference on Wave and Quantum Aspects of Contemporary Optics; (2003) https://doi.org/10.1117/12.545163
Event: 13th Polish-Czech-Slovak Conference on Wave and Quantum Aspects of contemporary Optics, 2002, Krzyzowa, Poland

Abstract

The application of fluorescence spectroscopy methods in investigations of camptothecin (CPT) is presented in this paper. Fluorescence of CPT enables one to follow the process of hydrolysis, i.e. the process of converting the biologically active lactone form into inactive carboxylate. The fluorescence spectra of CPT recorded during the hydrolysis were analysed using Principale Component Analysis and Factor Analysis. The results obtained on the basis of fluorescence spectra analysis are compared with HPLC data.

Citation Download Citation

Stefan Kruszewski, Ryszard Siuda, Blanka Ziomkowska, and Michal Cyrankiewicz "Kinetics of camptothecin hydrolysis determined by PCA and FA analysis of fluorescence spectra", Proc. SPIE 5259, 13th Polish-Czech-Slovak Conference on Wave and Quantum Aspects of Contemporary Optics, (21 November 2003); https://doi.org/10.1117/12.545163

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