Multichannel surface imaging and analysis of self-assembled monolayers and proteins

Hyeon-Bong Pyo; Yong-Beom Shin; Min-Gon Kim; Hyun C. Yoon

doi:10.1117/12.515543

15 March 2004 Multichannel surface imaging and analysis of self-assembled monolayers and proteins

Hyeon-Bong Pyo, Yong-Beom Shin, Min-Gon Kim, Hyun C. Yoon

Proceedings Volume 5261, Smart Medical and Biomedical Sensor Technology; (2004) https://doi.org/10.1117/12.515543
Event: Optical Technologies for Industrial, Environmental, and Biological Sensing, 2003, Providence, RI, United States

Abstract

Multichannel images of 11-Mercaptoundecanoic acid and 11-Mercapto-1-undecanol self-assembled monolayers (SAMs) together with a biospecific interferon-gamma (IFN-gamma)/anti-IFN-gamma antibody immunoreaction were observed by two-dimensional surface plasmon resonance (2D-SPR) imaging system. Patterning process for SAM was simplified by exploiting direct photooxidation of thiol bonding (photolysis) instead of conventional photolithography. Sharper images were resolved by using a white light source in combination with a narrow bandpass filter, minimizing the diffraction patterns on the images. The line profile calibration of the image contrast caused by different resonance conditions at each points on the sensor surface enabled us to discriminate the monolayer thickness in a sub-nanometer scale. For protein patterning, a precipitation scheme induced by biocatalytic reaction was implied for the signal amplification. Specific binding of IFN-gamma antigen with surface-immobilized antibody was found detectable down to the concentration of 1 ng/mL.

Citation Download Citation

Hyeon-Bong Pyo, Yong-Beom Shin, Min-Gon Kim, and Hyun C. Yoon "Multichannel surface imaging and analysis of self-assembled monolayers and proteins", Proc. SPIE 5261, Smart Medical and Biomedical Sensor Technology, (15 March 2004); https://doi.org/10.1117/12.515543

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KEYWORDS

Self-assembled monolayers

Proteins

Optical lithography

Bandpass filters

Calibration

Diffraction

Imaging systems

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