29 March 2004 Laser-tweezer-controlled solid immersion lens for high-resolution imaging in microfluidic and biological samples
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Abstract
A novel technique is presented which integrates the capacity of a laser tweezer to optically trap and manipulate objects in three-dimensions with the resolution-enhanced imaging capabilities of a solid immersion lens (SIL). Up to now, solid immersion lens imaging systems have relied upon cantilever-mounted SILs that are difficult to integrate into microfluidic systems and require an extra alignment step with external optics. As an alternative to the current state-of-art, we introduce a device that consists of a free-floating SIL and a laser optical tweezer. In our design, the optical tweezer, created by focusing a laser beam through high numerical aperture microscope objective, acts in a two-fold manner: both as a trapping beam for the positioning and alignment of the SIL and as an near-field scanning beam to image the sample through the SIL. Combining the alignment, positioning, and imaging functions into a single device allows for the direct integration of a high resolution imaging system into microfluidic and biological environments.
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Aaron L. Birkbeck, Sanja Zlatanovic, Mihrimah Ozkan, Sadik C. Esener, "Laser-tweezer-controlled solid immersion lens for high-resolution imaging in microfluidic and biological samples", Proc. SPIE 5275, BioMEMS and Nanotechnology, (29 March 2004); doi: 10.1117/12.522943; https://doi.org/10.1117/12.522943
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