29 March 2004 Laser-tweezer-controlled solid immersion lens for high-resolution imaging in microfluidic and biological samples
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Abstract
A novel technique is presented which integrates the capacity of a laser tweezer to optically trap and manipulate objects in three-dimensions with the resolution-enhanced imaging capabilities of a solid immersion lens (SIL). Up to now, solid immersion lens imaging systems have relied upon cantilever-mounted SILs that are difficult to integrate into microfluidic systems and require an extra alignment step with external optics. As an alternative to the current state-of-art, we introduce a device that consists of a free-floating SIL and a laser optical tweezer. In our design, the optical tweezer, created by focusing a laser beam through high numerical aperture microscope objective, acts in a two-fold manner: both as a trapping beam for the positioning and alignment of the SIL and as an near-field scanning beam to image the sample through the SIL. Combining the alignment, positioning, and imaging functions into a single device allows for the direct integration of a high resolution imaging system into microfluidic and biological environments.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Aaron L. Birkbeck, Aaron L. Birkbeck, Sanja Zlatanovic, Sanja Zlatanovic, Mihrimah Ozkan, Mihrimah Ozkan, Sadik C. Esener, Sadik C. Esener, } "Laser-tweezer-controlled solid immersion lens for high-resolution imaging in microfluidic and biological samples", Proc. SPIE 5275, BioMEMS and Nanotechnology, (29 March 2004); doi: 10.1117/12.522943; https://doi.org/10.1117/12.522943
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