Progress in directed energy control of vectors for microbes and other cells

Johnathan L. Kiel D.V.M.; Jill E. Parker; Eric A. Holwitt; Jeeva Vivekananda; Mark A. Sloan; Lucille J. V. Stribling

doi:10.1117/12.522922

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13 July 2004 Progress in directed energy control of vectors for microbes and other cells

Johnathan L. Kiel D.V.M., Jill E. Parker, Eric A. Holwitt, Jeeva Vivekananda, Mark A. Sloan, Lucille J. V. Stribling

Author Affiliations +

Proceedings Volume 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV; (2004) https://doi.org/10.1117/12.522922
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Biosynthetic semiconductor, diazoluminomelanin (DALM), is a polymer of tyrosine, luminol, and nitrite. DALM has a very large cross section of absorption for light from ultraviolet to radio frequencies. This polymer can be made efficiently in a genetically engineered E.coli, JM109/pIC2ORNR1.1 (ATCC# 69905). We have been pursuing ways to couple electromagnetic radiation to vectors using this polymer. DNA capture elements (DCEs; formerly aptamers) have made this possible. We incorporated DCEs into the plasmid of this E. coli to direct binding to whatever microbe or cell desired and to produce DALM attached to the plasmid DNA. Using two other vectors pSV₂neoNR10₁ or pSV₂neoNR800₅ (ATCC # 69617 and 69618, respectively), both propagated in the E. coli host HB101, we have also inserted genes necessary for DALM production into animal and human cell lines (mouse monocytic leukemia: ATCC # CRL- 11771, -11772, -1173, mouse mammary adenocarcinoma: ATCC# CRL-12184, -12185; and human carcinoma of the cervix: ATCC # CRL-12510). The DCE/DALM vectors can be used to tag target cells, detectable by broad-spectrum light absorbance, luminescence, or fluorescence. DCE/DALM can further be activated with light, microwave energy, or by oxidative chemistry to kill the targeted microbes or other cells.

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Johnathan L. Kiel D.V.M., Jill E. Parker, Eric A. Holwitt, Jeeva Vivekananda, Mark A. Sloan, and Lucille J. V. Stribling "Progress in directed energy control of vectors for microbes and other cells", Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); https://doi.org/10.1117/12.522922

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