14 June 2004 Chemiluminescence detection of reactive oxygen species during photodynamic therapy
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Abstract
Photodynamic therapy (PDT) utilizes photon energy to activate a pre-administered photosensitizer drug in tissue to achieve a localized tumor control. PDT cell killing mechanism is directly related to the reactive oxygen species (ROS) produced during the photochemical reactions. Conventional PDT dosimetry evaluates distributions of the photosensitizer drug, photon propagation and absorption, and availability of molecular oxygen in the target tissue. Yet, the ultimate bullet for the damaging effect is ROS. An evaluation of ROS production during PDT should provide a more direct marker for PDT. Fluoresceinyl Cypridina Luciferin Analog (FCLA) is a chemiluminescence probe that specifically interacts with ROS (singlet oxygen and/or superoxide). The work is a preliminary investigation of the feasibility using FCLA as a means to evaluate ROS production in PDT.
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Da Xing, Yanfang Qin, Yunxia Wu, Jin Zhou, Shiming Luo, Yonghong He, Qun Chen, "Chemiluminescence detection of reactive oxygen species during photodynamic therapy", Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); doi: 10.1117/12.528576; https://doi.org/10.1117/12.528576
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KEYWORDS
Photodynamic therapy

Oxygen

Chemiluminescence

Luminescence

Tissue optics

Microscopy

Analog electronics

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