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14 June 2004 Pulsed photodynamic inactivation of gram-negative bacteria: in vitro study using methylene blue
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Abstract
Pulsed photodynamic effect on Ps.aeruginosa has been investigated in vitro with the use of methylene blue (MB) as a photosensitizer. Bacterial suspensions were illuminated with 665-nm, nanosecond light pulses under the various drug and light dose conditions. Bacterial survival fraction decreased with increasing the drug and light doses, while no significant bacterial cell death was observed when the bacteria were washed after incubation with the drug. These results suggest a two-step photo-bactericidal mechanism; the photodynamic effect first induces membrane damage through which MB molecules were translocated into the bacterial cytoplasm, and then caused bacterial cell death.
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Masafumi Nagasawa, Shunichi Sato, Satoko Kawauchi, Nariyoshi Shinomiya, Daizo Saito, Hiroshi Ashida, and Minoru Obara "Pulsed photodynamic inactivation of gram-negative bacteria: in vitro study using methylene blue", Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); https://doi.org/10.1117/12.530817
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