Two-photon fluorescence biosensing with conventional and photonic crystal fibers

Mon Thiri Myaing; Jing Yong Ye; Theodore B. Norris; Thommey P. Thomas; James R. Baker Jr.; William J. Wadsworth; Geraud Bouwmans; Jonathan C. Knight; Philip St. J. Russell

doi:10.1117/12.529394

10 June 2004 Two-photon fluorescence biosensing with conventional and photonic crystal fibers

Mon Thiri Myaing, Jing Yong Ye, Theodore B. Norris, Thommey P. Thomas, James R. Baker Jr., William J. Wadsworth, Geraud Bouwmans, Jonathan C. Knight, Philip St. J. Russell

Author Affiliations +

Proceedings Volume 5317, Optical Fibers and Sensors for Medical Applications IV; (2004) https://doi.org/10.1117/12.529394
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Fluorescence is a powerful tool for biosensing, but conventional fluorescence measurements are limited because solid tumors are highly scattering media. To obtain quantitative in vivo fluorescence information from tumors, we have developed a two-photon optical fiber fluorescence (TPOFF) probe where excitation light is delivered and the two-photon fluorescence (TPF) excited at the tip of the fiber is collected back through the same fiber. In order to determine whether this system can provide quantitative information, we measured the fluorescence from a variety of systems including mouse tumors (both ex vivo and in vivo) which were transfected with the gene to express varying amounts of green fluorescence protein (GFP), and tumors which were labeled with targeted dendrimer-based drug delivery agents. The TPOFF technique showed results quantitatively in agreement with those from flow cytometry and confocal microscopy. In order to improve the sensitivity of our fiber probe, we developed a dual-clad photonic-crystal fiber which allowed single-mode excitation and multimode (high numerical aperture) collection of TPF. These experiments indicate that the TPOFF technique is highly promising for real-time, in vivo, quantitative fluorescence measurements.

Citation Download Citation

Mon Thiri Myaing, Jing Yong Ye, Theodore B. Norris, Thommey P. Thomas, James R. Baker Jr., William J. Wadsworth, Geraud Bouwmans, Jonathan C. Knight, and Philip St. J. Russell "Two-photon fluorescence biosensing with conventional and photonic crystal fibers", Proc. SPIE 5317, Optical Fibers and Sensors for Medical Applications IV, (10 June 2004); https://doi.org/10.1117/12.529394

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Tumors

In vivo imaging

Dendrimers

Single mode fibers

Photonic crystal fibers

Biosensing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years