Virus-host interaction probed by native Stokes shift fluorescence

Alvin Katz; Alexandra Alimova; Paul Gottlieb; Rakhi Podder; Glenn Minko; Hue Wei; Robert R. Alfano

doi:10.1117/12.528330

1 July 2004 Virus-host interaction probed by native Stokes shift fluorescence

Alvin Katz, Alexandra Alimova, Paul Gottlieb, Rakhi Podder, Glenn Minko, Hue Wei, Robert R. Alfano

Proceedings Volume 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies; (2004) https://doi.org/10.1117/12.528330
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The fluorescence spectra from Φ6 and Φ12 cystoviruses and their pseudomonad host were investigated. The predominant fluorophore in both the Φ6 and Φ12 viruses and pseudomonas syringae is tryptophan. The emission maxima of the virus emission was found to be at 330 nm, compared to an emission maxima from the bacteria, which typically varies between 337 and 349 nm, depending on growth conditions. This difference Stokes shifts between the viruses and syringae hosts are most likely due to the virus proteins being in a much less polar environment than the bacteria proteins. This difference in Stokes shift was used to monitor the infection process.

Citation Download Citation

Alvin Katz, Alexandra Alimova, Paul Gottlieb, Rakhi Podder, Glenn Minko, Hue Wei, and Robert R. Alfano "Virus-host interaction probed by native Stokes shift fluorescence", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); https://doi.org/10.1117/12.528330

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