1 July 2004 Lanthanide-enhanced luminescence (LEL) with one and two photon excitation of Quantum Dyes lanthanide(III)-macrocycles
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Improvements in the lanthanide enhanced luminescence (LEL) protocol have facilitated the use of the recently synthesized Eu(III)-macrocycle-mono-isothiocyanate, Quantum Dye, as a label. It was discovered that a homogeneous solution in ethanol or other solvent could be used to produce the lanthanide enhanced luminescence (LEL) effect, provided that the solution was permitted to evaporate. This protocol has been applied to the direct staining of cells in S phase, and was optimized for solid phase assays with Quantum Dye labeled streptavidin. Preliminary studies indicate that cells stained with the europium Quantum Dye can be observed both by conventional UV laser excitation and by infrared two-photon confocal microscopy. An enhancer has been found that enables the observation of simultaneous emissions from both the europium and terbium Quantum Dyes.
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Robert C. Leif, Margie C. Becker, Alfred J. Bromm, Nanguang Chen, Ann E. Cowan, Lidia M. Vallarino, Sean Yang, Robert M. Zucker, "Lanthanide-enhanced luminescence (LEL) with one and two photon excitation of Quantum Dyes lanthanide(III)-macrocycles", Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); doi: 10.1117/12.530284; https://doi.org/10.1117/12.530284

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