Highly efficient coherent anti-Stokes Raman scattering (CARS) microscopy

Thomas Hellerer; Annika M. K. Enejder; Ondrej Burkacky; Andreas Zumbusch

doi:10.1117/12.528265

21 June 2004 Highly efficient coherent anti-Stokes Raman scattering (CARS) microscopy

Thomas Hellerer, Annika M. K. Enejder, Ondrej Burkacky, Andreas Zumbusch

Proceedings Volume 5323, Multiphoton Microscopy in the Biomedical Sciences IV; (2004) https://doi.org/10.1117/12.528265
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Coherent Anti-Stokes Raman Scattering (CARS)-microscopy has in recent years developed as a promising microscopical technique for label-free microscopy of living cells. We propose a new concept, spectral focusing, for highly efficient coherent anti-Stokes Raman scattering (CARS) microscopy. It allows optimal use of the excitation energy of femtosecond laser pulses in terms of generated CARS signal against a low background. This is accomplished by introducing a linear chirp in the excitation pulses. The temporal delaying of the excitation pulses can be used to record vibrational spectra of a sample. Despite the inherently broad spectral width of the excitation pulses, the technique enables resolution of spectral features 60 times narrower than the bandwidth of the probe light. First applications of this technique are exemplified with CARS of micron sized crystallites of sodium nitroprusside, a commonly used hypotensive agent.

Citation Download Citation

Thomas Hellerer, Annika M. K. Enejder, Ondrej Burkacky, and Andreas Zumbusch "Highly efficient coherent anti-Stokes Raman scattering (CARS) microscopy", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); https://doi.org/10.1117/12.528265

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available