21 June 2004 Resonance-enhanced functional third harmonic optical microscopy
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For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.
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Shi-Wei Chu, Shi-Wei Chu, Szu-Yu Chen, Szu-Yu Chen, Tsung-Han Tsai, Tsung-Han Tsai, Tzu-Ming Liu, Tzu-Ming Liu, Shr-Bin Wu, Shr-Bin Wu, Sajal Biring, Sajal Biring, Juen-Kai Wang, Juen-Kai Wang, Yu-Lin Wang, Yu-Lin Wang, Kevin Chen, Kevin Chen, Bai-Lin Lin, Bai-Lin Lin, Chi-Kuang Sun, Chi-Kuang Sun, } "Resonance-enhanced functional third harmonic optical microscopy", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); doi: 10.1117/12.528130; https://doi.org/10.1117/12.528130

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