Microscopic tomography by digital interference holography

Myung K. Kim

doi:10.1117/12.524864

13 July 2004 Microscopic tomography by digital interference holography

Myung K. Kim

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.524864
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We are developing the digital interference holography (DIH) as a novel method of microscopic tomographic imaging by numerical superposition of a number of holographic fields taken with varying wavelengths. The digital interference holography does not involve pixel-by-pixel mechanical scanning of three-dimensional volume and yet achieves high lateral and longitudinal resolutions. The holographic interference pattern of an object is generated optically and recorded digitally using a CCD camera. The hologrpahic image field is numerically calculated using basic diffraction formulas and the process is repeated for a range of varying wavelengths at regular intervals. Numerical superposition, or digital interference, of the holographic image fields yields the desired three-dimensional representation of the object. Experiments have demonstrated a few-micron lateral and axial resolutions. Furthermore, since the DIH is a coherent imaging system, one can form true tomographic images of sub-surface structures, in the presence of diffuse scattering from overlying layers. By being able to generate true tomographic images of subsurface structures, without the need for three-dimensional mechanical scannig, the DIH method can provide a very efficient and versatile imaging modality for a wide range of applications in materials science and biomedical imaging.

Citation Download Citation

Myung K. Kim "Microscopic tomography by digital interference holography", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.524864

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KEYWORDS

Holography

Digital holography

Holograms

Image processing

3D image reconstruction

3D image processing

Tomography

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