13 July 2004 Mueller polarimetric microscopy
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Abstract
We present a multispectral polarimetric imaging system well suited for complete Mueller matrix microscopy. The source is a spectrally filtered halogen light bulb, and the image is formed on a fast CCD camera The light polarization is modulated before the sample and analyzed after the sample by using nematic liquid crystal modulators.. The whole Mueller matrix image of the sample is typically measured over 5 seconds for a good signal-to-noise ratio. The instrument design, together with an original and easy-to-operate calibration procedure provides a high polarimetric accuracy over wide ranges of wavelengths and magnifications. Mueller polarimetry provides separate images of scalar and vector retardation and dichroism of the sample, together with its depolarizing power, while all these effects do contribute simultaneously to the contrasts observed in standard polarized microsopy. Polarimetric images of several samples, namely an unstained rabbit cornea, a picrosirius red stained hepatic biopsy, and a rat artery specifically stained for collagen III are shown and discussed
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Blandine Laude-Boulesteix, Blandine Laude-Boulesteix, Antonello De Martino, Antonello De Martino, Gilles Le Naour, Gilles Le Naour, Catherine Genestie, Catherine Genestie, Laurent Schwartz, Laurent Schwartz, Enric Garcia-Caurel, Enric Garcia-Caurel, Bernard Drevillon, Bernard Drevillon, } "Mueller polarimetric microscopy", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); doi: 10.1117/12.527975; https://doi.org/10.1117/12.527975
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