Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

SeugnWoo Lee; Dong-Kyun Kang; HongKi Yoo; Dae-Gab Gweon; Suk-Won Lee; Kwang-Soo Kim

doi:10.1117/12.529884

13 July 2004 Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

SeugnWoo Lee, Dong-Kyun Kang, HongKi Yoo, Dae-Gab Gweon, Suk-Won Lee, Kwang-Soo Kim

Author Affiliations +

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.529884
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We describe the design and the implementation of reflection confocal scanning microscopy (CSM) using an acousto-optical deflector (AOD) for the fast horizontal scan and a galvanometer mirror (GM) for the slow vertical scan. In the beam scanning system it is important to maintain the lateral and the axial performance during scanning operation. We propose a simple method to design a scanning system using the finite ray tracing and the diffraction theory. We define a cost function which contains the effect of aberrations on the performance of microscopy. We construct the designed system and evaluate its performance. The OSLO simulation shows that the performances of CSM are not changed with deflection angle. So we conclude that the beam scanning system is properly designed. In addition, we propose an image formation method and show images obtained with the system.

Citation Download Citation

SeugnWoo Lee, Dong-Kyun Kang, HongKi Yoo, Dae-Gab Gweon, Suk-Won Lee, and Kwang-Soo Kim "Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.529884

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