13 July 2004 Enhancement of spectral change from change of cell nucleus size distribution in epithelium using multiclass linear discriminant
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Abstract
Here, we propose a new method to enhance the sensitivity of the reflectance spectrum to the scattering feature of the superficial tissue layer. This method is based on multiple discriminant analysis (MDA) in the eigen subspace of the spectrum. Considering the application of scattering imaging, we evaluated this method by performing multispectral imaging of two-layered tissue phantoms. A color map converted from the spectral reflectance well corresponds to variations in the size of the scatter in the first layer. In order to confirm our proposed method works well under more realistic conditions, we performed the computational simulations of the light propagation in the tissue. We used the simulation model combined with the Monte Carlo and the Mie scattering. Its conditions like the slab geometry and the particle distribution of the cell nucleus were estimated by the image measuring of pathological slices. Results on simulations show the possibility of enhancing the sensitivity of the reflectance spectrum to the scattering feature of the superficial tissue layer.
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Kazuhiro Gono, Kazuhiro Gono, Makoto Igarashi, Makoto Igarashi, Takashi Obi, Takashi Obi, Masahiro Yamaguchi, Masahiro Yamaguchi, Nagaaki Ohyama, Nagaaki Ohyama, } "Enhancement of spectral change from change of cell nucleus size distribution in epithelium using multiclass linear discriminant", Proc. SPIE 5326, Optical Biopsy V, (13 July 2004); doi: 10.1117/12.528902; https://doi.org/10.1117/12.528902
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