16 June 2004 Metallic nanostructures for plasmonic sensors using surface-enhanced fluorescence and Raman detection
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Abstract
We present the fabrication and characterization of silver island films with SiO2 coatings for application in plasmonic sensors based on surface-enhanced fluorescence and Raman detection. The emission spectral properties of goat anti-mouse immunoglobulin (IgG) F(ab')2 labeled with one or two fluorescein residues were examined on substrates with metallic silver islands. The self-quenching of fluorescein emission was mostly eliminated when this antibody fragment was held 60-90 Å from the surface of metallic silver islands, and our preliminary experiments demonstrated an 8-fold emission intensity increase. Similar surfaces were also examined for surface-enhanced Raman analysis of Rhodamine 123, a potential drug for photodynamic therapy.
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Fei Yan, Fei Yan, Musundi B. Wabuyele, Musundi B. Wabuyele, Guy D. Griffin, Guy D. Griffin, Tuan Vo-Dinh, Tuan Vo-Dinh, } "Metallic nanostructures for plasmonic sensors using surface-enhanced fluorescence and Raman detection", Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); doi: 10.1117/12.542455; https://doi.org/10.1117/12.542455
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