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16 June 2004 Nanostructured thin films for fluorescence enhancement
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Fluorescence is widely used as a spectroscopic tool or for biomedical imaging, in particular for DNA chips. Nanostructured metallic substrates permit to locally enhance the fluorescence signal which offer the possibility both to detect very small fluorophore concentrations and to trace precisely the bio-markers. We have developed substrates made of silver or gold nanoparticles covered with a spacer layer of alumina. Double metallic and dielectric gradients permit to directly map the fluorescence enhancement factor and to determine the best condition for maximum enhancement. One and two photons excitations are studied. Fluorescence enhancement reaches two orders of magnitudes. Lifetime measurements reveal additional information on the decay channels induced by the nanoparticle presence.
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Sandrine Leveque-Fort, Emmanuel Fort, Eric Le Moal, Jean-Pierre Lacharme, Marie-Pierre Fontaine-Aupart, and Christian Ricolleau "Nanostructured thin films for fluorescence enhancement", Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); doi: 10.1117/12.529909;

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