22 June 2004 Demonstration of optical microfluidics
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We demonstrate a novel method for the control of small droplets using laser-based heating. Temperature dependent interfacial surface tensions were the primary force used to move droplets. With this approach, ~1.7 μL to 14 pL droplets were moved on a bare, unmodified polystyrene surface, at speeds of up to 3 mm/s. Upon contact, droplets spontaneously fused and rapidly mixed within 33 ms. We performed an optical absorption-based protein assay using horseradish peroxidase and a chromogenic substrate (ABTS), and readily detected as little as ~125 attomoles of reacting enzyme.
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Kenneth T. Kotz, Konstantinos S. Kalogerakis, Kyle A. Noble, Sarah E. Smith, Gregory W. Faris, "Demonstration of optical microfluidics", Proc. SPIE 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, (22 June 2004); doi: 10.1117/12.538890; https://doi.org/10.1117/12.538890

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