Microelectronic electroporation array

Lee J. Johnson; Kara J. Shaffer; Perry Skeath; Frank Keith Perkins; Joseph Pancrazio; Dean Scribner

doi:10.1117/12.531843

22 June 2004 Microelectronic electroporation array

Lee J. Johnson, Kara J. Shaffer, Perry Skeath, Frank Keith Perkins, Joseph Pancrazio, Dean Scribner

Proceedings Volume 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II; (2004) https://doi.org/10.1117/12.531843
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Gene Array technology has allowed for the study of gene binding by creating thousands of potential binding sites on a single device. A limitation of the current technology is that the effects of the gene and the gene-derived proteins cannot be studied in situ the same way, thousand site cell arrays are not readily available. We propose a new device structure to study the effects of gene modification on cells. This new array technology uses electroporation to target specific areas within a cell culture for transfection of genes. Electroporation arrays will allow high throughput analysis of gene effects on a given cell's response to a stress or a genes ability to restore normal cell function in disease modeling cells. Fluorescent imaging of dye labeled indicator molecules or cell viability will provide results indicating the most effective genes. The electroporation array consists of a microelectronic circuit, ancillary electronics, protecting electrode surface for cell culturing and a perfusion system for gene or drug delivery. The advantages of the current device are that there are 3200 sites for electroporation, all or any subsets of the electrodes can be activated. The cells are held in place by the electrode material. This technology could also be applied to high throughput screening of cell impermeant drugs.

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Lee J. Johnson, Kara J. Shaffer, Perry Skeath, Frank Keith Perkins, Joseph Pancrazio, and Dean Scribner "Microelectronic electroporation array", Proc. SPIE 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, (22 June 2004); https://doi.org/10.1117/12.531843

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KEYWORDS

Electrodes

Microelectronics

Glasses

Multiplexers

Silicon

Tissues

Metals

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