14 June 2004 Improvement of sensitivity and spatial resolution for in vivo imaging with low-level light emitting probes by optical clearing method
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Abstract
Low-level light-emitting imaging technique often detects the light emerged at the tissue surface that is generated internally from a specific target. However, in most cases, the high scattering nature of biological tissue limits the sensitivity and spatial resolution of this imaging modality. In this paper, we report that a significant improvement of chemiluminescence (CL) imaging performance in terms of both sensitivity and spatial resolution can be achieved by use of the topical application of glycerol solution onto tissue sample, i.e. optical clearing approach. Monte Carlo simulation of internally-launched point source shows that the decrease of scattering coefficient of turbid medium, which can be achieved by optical tissue clearing approach, causes stronger peak intensity with a narrower full width at half maximum. The improvement becomes more significant with the source depth increasing from 1 mm to 5 mm. The experimental results shows tissue clearing with 50% glycerol solution could largely improve the brightness and the spatial resolution of CL imaging when the target is covered with biological tissue having thickness of 1mm or 3mm. This method could have potential application in the in vivo low-level light imaging technique.
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Ruikang K. Wang, Yonghong He, "Improvement of sensitivity and spatial resolution for in vivo imaging with low-level light emitting probes by optical clearing method", Proc. SPIE 5329, Genetically Engineered and Optical Probes for Biomedical Applications II, (14 June 2004); doi: 10.1117/12.529620; https://doi.org/10.1117/12.529620
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