1 June 2004 Simultaneous visualization of contact microscopic image and energetic charged particle tracks and its application to medicine
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Contact microscopy, enables us to visualize the detailed internal structure of biological cells. Exposure of biological specimen mounted on X-ray or UV sensitive materials to soft X-ray or UV introduces chemical damage to the materials, and the damage distribution due to the absorption of the X-rays or the UV by the specimen reveals as relief on the surface of the material after development process. The relief can be visualized with an AFM at high resolution of ~100 nm. We have applied the contact microscopy technique to high-resolution neutron-induced alpha-autoradiography for boron imaging in boron neutron capture therapy (BNCT). In BNCT, energetic alpha/lithium particles (range ~ single cell) from boron-neutron reactions introduce lethal damage to tumor cells selectively through thermal neutron irradiation with tumor-accumulating boron compounds. To understand the mechanism of drug delivery of those boron compounds is significant to evaluate the efficacy of BNCT. In the new technique, we can visualize those alpha/lithium particle tracks as etch pits and contact X-ray/UV microscopic image of tumor cells as relief on the surface of CR-39 plastic track detectors after etching process. Achievable resolution was ~100 nm with AFM readout, so that we can perform the boron imaging at subcellular scale.
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Kuniaki Amemiya, Kuniaki Amemiya, Hiroyuki Takahashi, Hiroyuki Takahashi, Toru Naruse, Toru Naruse, Masaharu Nakazawa, Masaharu Nakazawa, Hironobu Yanagie, Hironobu Yanagie, Tomoyuki Hisa, Tomoyuki Hisa, Masazumi Eriguchi, Masazumi Eriguchi, Yoshinobu Nakagawa, Yoshinobu Nakagawa, Toshikazu Majima, Toshikazu Majima, Teruyoshi Kageji, Teruyoshi Kageji, Yoshinori Sakurai, Yoshinori Sakurai, Tooru Kobayashi, Tooru Kobayashi, Teruaki Konishi, Teruaki Konishi, Kotaro Hieda, Kotaro Hieda, Nakahiro Yasuda, Nakahiro Yasuda, Koichi Ogura, Koichi Ogura, } "Simultaneous visualization of contact microscopic image and energetic charged particle tracks and its application to medicine", Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); doi: 10.1117/12.528777; https://doi.org/10.1117/12.528777

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