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23 December 2003 Microfabricated polymer filter device for bio-applications
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Proceedings Volume 5345, Microfluidics, BioMEMS, and Medical Microsystems II; (2003)
Event: Micromachining and Microfabrication, 2004, San Jose, California, United States
The need for disposable diagnostic sensors in the health care industry has been a major driver in the development of low-cost polymer microfluidic devices. Of crucial importance to many of these devices is the incorporation of sieves and filters for the pretreatment of biological samples. Much of the previous work on integrating filtration systems in microdevices has focused on silicon and glass technologies. Of more difficulty, due to the different manufacturing methodology and lower mechanical strength, is the integration of filtration systems in polymer microfluidic chips. This paper presents a design and construction methodology to fabricate such integrated devices in polyethylene terepthalate (PET) and describes their characterization for particle filtration. To demonstrate the application of these systems, DNA extraction from whole blood was investigated. This currently represents a major stumbling block for point-of-care diagnostics. To this end two approaches were taken; the isolation of leucocytes for subsequent DNA extraction, and the trapping of silica microspheres for DNA adsorption. The polymer surfaces of the fluidic chips were modified by UV exposure and chemical etching to increase their surface energy for improved non-specific binding and electroosmotic flow characteristics. Integrated filtration devices were successfully fabricated with excimer laser machined membranes having pore dimensions down to 1μm, and contact angles from 75° down to less than 25° were achieved using UV modification, and from 75° down to 16° by chemical modification of PET. White blood cells were filtered from whole blood and silica particle retention was demonstrated successfully.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Micah Atkin, Karl Poetter, Robert Cattrall, and Erol Harvey "Microfabricated polymer filter device for bio-applications", Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003);

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