Development of a percutaneous optical imaging system for tracking vascular gene expression: a feasibility study using human tissuelike phantoms

Sourav Kumar Kar; Ananda Kumar; Xiaoming Yang

doi:10.1117/12.535598

5 May 2004 Development of a percutaneous optical imaging system for tracking vascular gene expression: a feasibility study using human tissuelike phantoms

Sourav Kumar Kar, Ananda Kumar, Xiaoming Yang

Author Affiliations +

Proceedings Volume 5367, Medical Imaging 2004: Visualization, Image-Guided Procedures, and Display; (2004) https://doi.org/10.1117/12.535598
Event: Medical Imaging 2004, 2004, San Diego, California, United States

Abstract

Noninvasive tracking of vascular gene delivery and expression forms an important part of successfully implementing vascular gene therapy methods for the treatment of atherosclerosis and various cardiovascular disorders. While ultrasound and MR imaging have shown promise in the monitoring of gene delivery to the vasculatures, optical imaging has shown promise for tracking gene expression. Optical imaging using bioreporter genes like Green Fluorescent Protein (GFP), Red Fluorescent Protein (RFP) and Luciferase to track and localize the therapeutic gene have helped provide an in vivo detection method of the process. The usage of GFP and RFP entails the detection of the fluorescent signal emitted by them on excitation with light of appropriate wavelength. We have developed a novel percutaneous optical imaging system that may be used for in vivo tracking vascular fluorescent gene expression in deep-seated vessels. It is based on the detection of the fluorescent signal emitted from GFP tagged cells. This phantom study was carried out to investigate the performance of the optical imaging system and gain insights into its performance record and study improvisation possibilities.

Citation Download Citation

Sourav Kumar Kar, Ananda Kumar, and Xiaoming Yang "Development of a percutaneous optical imaging system for tracking vascular gene expression: a feasibility study using human tissuelike phantoms", Proc. SPIE 5367, Medical Imaging 2004: Visualization, Image-Guided Procedures, and Display, (5 May 2004); https://doi.org/10.1117/12.535598

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KEYWORDS

Optical imaging

Green fluorescent protein

Tissue optics

Imaging systems

Luminescence

Capillaries

Absorption

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