Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

Mohamed-Adel Slamani; Andrzej Krol; Jacques Beaumont; Robert L. Price; Ioana L. Coman; Edward D. Lipson

doi:10.1117/12.536232

12 May 2004 Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

Mohamed-Adel Slamani, Andrzej Krol, Jacques Beaumont, Robert L. Price, Ioana L. Coman, Edward D. Lipson

Proceedings Volume 5370, Medical Imaging 2004: Image Processing; (2004) https://doi.org/10.1117/12.536232
Event: Medical Imaging 2004, 2004, San Diego, California, United States

Abstract

Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

Citation Download Citation

Mohamed-Adel Slamani, Andrzej Krol, Jacques Beaumont, Robert L. Price, Ioana L. Coman, and Edward D. Lipson "Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy", Proc. SPIE 5370, Medical Imaging 2004: Image Processing, (12 May 2004); https://doi.org/10.1117/12.536232

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