12 May 2004 Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy
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Abstract
Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.
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Mohamed-Adel Slamani, Mohamed-Adel Slamani, Andrzej Krol, Andrzej Krol, Jacques Beaumont, Jacques Beaumont, Robert L. Price, Robert L. Price, Ioana L. Coman, Ioana L. Coman, Edward D. Lipson, Edward D. Lipson, } "Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy", Proc. SPIE 5370, Medical Imaging 2004: Image Processing, (12 May 2004); doi: 10.1117/12.536232; https://doi.org/10.1117/12.536232
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