DNA capture elements for rapid detection and identification of biological agents

Johnathan L. Kiel D.V.M.; Jill E. Parker; Eric A. Holwitt; Jeeva Vivekananda

doi:10.1117/12.532043

13 August 2004 DNA capture elements for rapid detection and identification of biological agents

Johnathan L. Kiel D.V.M., Jill E. Parker, Eric A. Holwitt, Jeeva Vivekananda

Proceedings Volume 5416, Chemical and Biological Sensing V; (2004) https://doi.org/10.1117/12.532043
Event: Defense and Security, 2004, Orlando, Florida, United States

Abstract

DNA capture elements (DCEs; aptamers) are artificial DNA sequences, from a random pool of sequences, selected for their specific binding to potential biological warfare agents. These sequences were selected by an affinity method using filters to which the target agent was attached and the DNA isolated and amplified by polymerase chain reaction (PCR) in an iterative, increasingly stringent, process. Reporter molecules were attached to the finished sequences. To date, we have made DCEs to Bacillus anthracis spores, Shiga toxin, Venezuelan Equine Encephalitis (VEE) virus, and Francisella tularensis. These DCEs have demonstrated specificity and sensitivity equal to or better than antibody.

Citation Download Citation

Johnathan L. Kiel D.V.M., Jill E. Parker, Eric A. Holwitt, and Jeeva Vivekananda "DNA capture elements for rapid detection and identification of biological agents", Proc. SPIE 5416, Chemical and Biological Sensing V, (13 August 2004); https://doi.org/10.1117/12.532043

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